Moe Mahmoud

5 Questions 1 Answers 0 Followers

Questions related from Moe Mahmoud

How do I over-express a protein ?

Hi Guys, hope all is well with you. I want to overexpress a protein using HEK 293 Cell line. Could you please kindly tell me what is the most convenient way to do so. I read something about...

02 November 2014 507 1 View

How to handle a frozen PVDF membrane, which hasn't been treated with any antibodies yet ?

Hi guys, so after blotting the membrane, and checking for total protein content, I washed the membrane for 20 mins in blocking buffer to remove ponceau s stain, then washed in distilled water for...

10 October 2014 9,928 3 View

What happens if the beads volume used in Immunoprecipitation was lower than suggested ?

If the beads volume used was lower than suggested (normal), would the immunoprecipitation not work at all, or it will work but the amount of protein precipitated will decrease, and hence fainter...

07 October 2014 2,812 2 View

Why does my dye front travel during PAGE at differing speeds ?

Hi guys, so while carrying out PAGE, I face a weird issue (happened with different gel concentrations), my dye front sometimes doesn't travel at the same speed through the gel, meaning the first...

25 September 2014 528 5 View

Can anyone help with the calculation of the protein sample volume before electrophoresis?

Hi, hope all is well with you guys. I have a question regarding the calculation of the volume of the protein sample prior to the loading for electrophoresis. I always make sure to load the same...

05 September 2014 5,216 5 View