Mj Bugay

3 Questions 2 Answers 0 Followers

Questions related from Mj Bugay

Experience with changing thermocyclers DURING PCR?

Hi everyone, I was running a PCR and, unfortunately, the thermocycler died around the 7th cycle. It felt like a waste to simply throw away the PCR, so I put it on another thermocycler and lowered...

30 October 2024 6,221 4 View

Low concentration after index PCR - troubleshooting suggestions?

Hello. I'm looking for suggestions on troubleshooting index (or barcode) PCR. Specifically, my problem is that some of my sample DNA concentrations dropped after adding the indices/barcodes. My...

18 September 2024 7,810 2 View

Why is there a difference between sample DNA concentration between Qubit Broad Range and High Sensitivity?

With the Qubit High Sensitivity DNA kit, I was measuring 8 samples (2uL each + 198 of the working solution), and noticed that 4 of the 8 samples had readings of >60ng/uL. I then re-did the...

23 April 2024 7,225 1 View