Mitchell Bryant

2 Questions 1 Answers 0 Followers

Questions related from Mitchell Bryant

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

17 February 2021 398 4 View

Gene of interest is in reverse orientation. Will this cause KO issues?

I am currently designing my CRISPR protospacers and choosing my PAM sites so that I can knock out a gene from a fungal organism. I found the sequence of my gene of interest from FungiDB (NCBI...

26 March 2020 5,642 2 View