Mita Tran

5 Questions 5 Answers 0 Followers

Questions related from Mita Tran

Unexpected Multiple Bands After Plasmid Linearization: Seeking Insights on Gel Electrophoresis Results ?

I have a plasmid (~9.3kb) with a small 20 nt insert, which I created using the Q5 Site-Directed Mutagenesis Kit via PCR. Before transformation, I obtained a clear band at 9.3 kb. I picked up a...

14 August 2024 5,543 3 View

Why were cDNA PCR got multiple bands when added DMSO?

Dear all, All samples are cDNA, using reverse transcriptase enzyme to make cDNA from RNA. One group is doing PCR without DMSO, and other group is doing PCR with DMSO. One is without DMSO, the...

05 October 2020 1,581 3 View

CDNA PCR problems: why did the sample without reverse transcriptase enzyme (band 5 in both two genes) have strong band with gen A?

Dear all, These are data from gel base of cDNA sample for PCR with protocol attached on the figure. Band 5 is no reverse transcriptase enzyme during doing cDNA library used random primer. With gen...

05 October 2020 9,030 4 View

Deal with RRBS data

Dear all scientists, I am dealing with RRBS data. I used VIM to read big data. Data are Methylation level (%) of both CpG sites and other couple nucleotide sites. I only want to get CpG sites not...

01 January 1970 9,795 0 View

Unexpected Multiple Bands After Plasmid Linearization: Seeking Insights on Gel Electrophoresis Results

I have a plasmid (~9.3kb) with a small 20 nt insert, which I created using the Q5 Site-Directed Mutagenesis Kit via PCR. Before transformation, I obtained a clear band at 9.3 kb. I picked up a...

01 January 1970 4,486 1 View