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Hello I tried to make perforated patch recordings of cultured neurons with amphotericin B. With only a few cells I could get R > 1 GOhm. Usually resistance rises up to 200-600 MOhm and then...
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I have a protein normally localized on cell membrane (in HEK cells), with a transmembrane domain. How can I target this protein into ER membrane and make it to accumulate there?
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