Miriam Funes

2 Questions 4 Answers 0 Followers

Questions related from Miriam Funes

How to differentiate hMSC into chondrocytes in a 2D culture?

I have seen the protocols for chondrogenesi in a 3D culture (micromass) but I would like to know if there is a protocol for culturing the cells in a monolayer and adding some factors so they can...

05 May 2014 6,282 6 View

Could kozak sequence mutation be the reason for not having expression of a cloned gene in S.cerevisiae?

I am trying to express a receptor (G-coupled protein receptor) in the membrane of S.cerevisiae. I have cloned the gene in a pITy plasmid, but after check the sequence I discovered a mutation in...

12 December 2012 7,146 9 View