A problem with generation of lentiviral particles of a large gene (4kb)?

I am trying to generate lentiviral particles from a insert (4Kb) cloned into a lentiviral vector (pWPI, 11Kb) and I am having some issues. I transfected HEK 293T cells with the pMD2G, pPAX and our...

03 August 2018 5,259 3 View