Miri Hillel

4 Questions 3 Answers 0 Followers

Questions related from Miri Hillel

Why qPCR protocols suggest using 2x primers and 1x probe?

hi all- it might be a very basic question but i struggle with the rational idea in my head- when performing qPCR we need F primer, R primer and a probe (taq-based qPCR). i usually use the same...

01 May 2022 7,012 2 View

Off target crispr cut tool?

Does anyone familiar with an online software in which I can insert my gRNA seq and it will show me off targets genomic areas than might get cut as well? I mean other than Benchling..... Thanks, Miri

31 May 2018 1,956 2 View

When linearizing a plasmid pre-transfection, do I need to dephosphorilate it as well?

I an recently trying to transfect 293T cells with a plasmid which should utilize as a homolog sequence for HDR in genome editing via CRISPR/Cas9. since this is not working for me for quite a while...

28 February 2017 2,093 2 View

T-cell stimulation with PHA-L: What am I doing wrong?

Hi, I'm trying to set an assay of cell stimulation with PHA-L and it's just won't work! I usualy take heparinized whole blood and incubate it for 24hr in 37oC 5% CO2 with 5ug/ml PHA-L following...

16 July 2014 8,531 5 View