Minh T. Le

3 Questions 6 Answers 0 Followers

Questions related from Minh T. Le

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

27 June 2019 9,127 2 View

What does an extra, unclear band coupled with a clear band indicates in gel electrophoresis?

Hello, I'm using PCR to assess the differences between the banding patterns of a microsatellite in different salamander species. In my resulting gel, there's extra unclear bands underneath the...

22 December 2018 8,791 6 View

How to distinguish between 2 alleles with similar bp sizes in gel electrophoresis?

Hello, What technique or modification to the gel electrophoresis procedure do I need in order to increase the difference in distance travelled by two alleles with very similar sizes? In my gel...

22 December 2018 2,337 2 View