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Why are qPCR amplicon about 100bp? Can PCR products improve the sensitivity of PCR? PS: I do amplify the 16S of environmental sample.
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Recently, I took some deep sea samples and extracted DNA from the sample. I want to know the abundance of the community (bacteria and archaea). So I tried to do the qPCR. When I search the...
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I extract DNA from the sediment sample, but the ratio of 260/230 is much lower than 1.7. I use the Nanodrop to measure the ratio. I use some methods such as cold ethanol washing or phenol...
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