Ming Yang

4 Questions 7 Answers 0 Followers

Questions related from Ming Yang

Question on b y ions：Why is a peptide framented only once in collision cell,eg: HCD, in the mass spec?

Dear Experts: We know that only when a peptide is fragmented only once meaning that a precursor is breaked only once to form two parts that the b y ions nomenclature can be true. But I think the...

09 September 2017 2,344 6 View

Help:Most Spectra with clear reporter ions were identified as non-labled peptides. Why?????

Recently I am facing a low labeling efficiency problem in iTRAQ experiment. The key point is, when I went to check the MS2 spectra of the non-labled peptides PD1.4 gived, I found most of them have...

09 September 2017 7,134 3 View

Any ideas for the early elution of peptides which means that most of the peptides are eluted in the 15- 35min range in a 120min LC-MS proteomics run?

Just as showed in attached picture, in a 120min LC-MS run, there is a high and wide peak group in about 15-30min and in the rest of the elution time, the peak intensity is low which means that...

09 September 2017 8,279 7 View

Why since the iTRAQ is sensitive to water but peptides are resusopended in 0.5M TEAB water solution, Why??

The iTRAQ reagent is very sensitive to water so that is why after taking the iTRAQ reagent vials out of the fridge we need to let it reach to the room temperature to avodi the water in air...

08 August 2017 4,932 3 View