Ming Zhou

4 Questions 6 Answers 0 Followers

Questions related from Ming Zhou

Why did I get so many negative clone after electronic shock when constructing a phage library?

I use phagemid pCDisplay3 and M13KO7 to construct Human Naive phage library. before digested with SfiI , I prepared ScFv (750bp) using gel purification. After electronic shock , ,plating on...

27 March 2020 4,130 2 View

Why did it produce more M13KO7 than displayed phages in phage library production?

When I did the phage library production, it was observed that there were 10-fold M13KO7 phage particle than phagemid-packaged phage in my phage library. As we all knew, when phagemid and...

22 November 2019 2,848 0 View

Why do I get a high titer on Kana plate after biopaning?

I performed a biopaning and elute the phage using 0.1M Gly-HCL pH2.2, after nuetralizaiton , phage titer was done. And I plate the TG1 ,infected with eluted library, on Kana and Amp plate ,...

19 November 2019 7,556 4 View

What will influence the efficiency that phage in fect TG1 ?

I do serial dilution of phage display to 10e10, then infect TG1 at 37℃ for 30min, the plate the mixture on 2*YTA, incubate at 37℃ overnight . However, I do not find any colony on plate...

01 March 2019 9,333 5 View