Ming Zhou

2 Questions 4 Answers 0 Followers

Questions related from Ming Zhou

Why did I get so many negative clone after electronic shock when constructing a phage library?

I use phagemid pCDisplay3 and M13KO7 to construct Human Naive phage library. before digested with SfiI , I prepared ScFv (750bp) using gel purification. After electronic shock , ,plating on...

27 March 2020 4,106 2 View

What will influence the efficiency that phage in fect TG1 ?

I do serial dilution of phage display to 10e10, then infect TG1 at 37℃ for 30min, the plate the mixture on 2*YTA, incubate at 37℃ overnight . However, I do not find any colony on plate...

01 March 2019 9,325 5 View