Min Han Lew

4 Questions 8 Answers 0 Followers

Questions related from Min Han Lew

Can anyone recommend a good molecular docking simulator used for prediction of allergen-antibody interactions?

Molecular docking simulation is one of the computational techniques commonly used to predict the biological interaction between molecules. I am currently studying on the molecular interactions...

05 May 2014 9,143 3 View

What strategy is better for elution during Ni-NTA affinity purification, imidazole or pH gradient?

Imidazole and pH gradient are the two most common strategies used in the Ni-NTA purification of his-tagged recombinant proteins. Imidazole is the competitive inhibitor for histidine-tagged...

05 May 2014 9,877 7 View

Can anyone help with measurement of protein refolding properties by UV visible spectroscopy?

UV visible spectroscopy are one of the alternative method used to measure the folding and kinetics of the protein. As my recombinant protein is expressed in the form of inclusion bodies, I would...

02 February 2014 5,229 7 View

What is the effect of glycerol on mice immunization?

I am planning to immunize the Balb/c mice using the recombinant protein for generation of antigen specific antibody used for ELISA assay. The his-tagged recombinant protein was purified using...

01 January 2014 5,489 5 View