Min Wei

2 Questions 3 Answers 0 Followers

Questions related from Min Wei

Can we use clone a gene into gateway destination vector via restriction enzyme cloning?

Have anyone tried to clone a gene into gateway destination vector via restriction enzyme cloning? I know LR cloning is good but the LR cloning kit is kind of expensive to our lab. So I was...

08 August 2021 1,039 4 View

What are the difference between selectable markers and bacterial resistance?

For example, the pPGKpuro from Addgene has a selectable marker, puromycin and a bacterial resistance, Ampicillin. According to my understanding, this plasmid confers resistance to both ampicillin...

21 June 2017 7,747 2 View