Min Han Lew

3 Questions 6 Answers 0 Followers

Questions related from Min Han Lew

Is the glutathione redox reaction necessary for in vitro protein refolding?

For in vitro protein refolding, the addition of the reduced and oxidized glutathione (-GSH- and -GSSG-) is useful to facillitate the disulfide bond formation of the protein. However, some...

09 September 2014 7,973 6 View

What strategy is better for elution during Ni-NTA affinity purification, imidazole or pH gradient?

Imidazole and pH gradient are the two most common strategies used in the Ni-NTA purification of his-tagged recombinant proteins. Imidazole is the competitive inhibitor for histidine-tagged...

09 May 2014 2,913 6 View

What is the effect of glycerol on mice immunization?

I am planning to immunize the Balb/c mice using the recombinant protein for generation of antigen specific antibody used for ELISA assay. The his-tagged recombinant protein was purified using...

04 January 2014 5,269 4 View