Min Cheol Kim

3 Questions 1 Answers 0 Followers

Questions related from Min Cheol Kim

How can you quantify proteins eluted by 500mM imidazole?

Hello. I am a graduate student making recombinant proteins at university. Recently, I have succeeded in the expression of recombinant proteins and purification and refolding using Ni-NTA, and...

13 October 2022 6,245 6 View

How to identify refolding protein?

Hello. I am a graduate student making recombinant proteins at university. Recently, I have succeeded in the expression of recombinant proteins and purification and refolding using Ni-NTA, and I...

11 October 2022 2,393 3 View

Can I add additional ingredients to the lysis buffer?

hello. I am a graduate student making recombinant proteins at university. Once the recombinant protein expression was successful. Additionally, I plan to proceed with affinity chromatography...

29 August 2022 9,633 3 View