Why vitrified mouse oocytes lyse at 0.25 M sucrose after undergone several steps of thawing from 0.75M and 0.5M sucrose solution?

We followed the oocyte vitrification and thawing procedure based on Zhang et al (2016) on inbred mouse oocytes. Unfortunately, after post-thawing, the oocytes remain lysed at 0.25 mol/L several...

14 September 2022 6,278 0 View