7 Questions 13 Answers 0 Followers
Questions related from Mildred A Iro
I am looking to investigate miRNAs to compare expression between patients and healthy controls. The patients are likely to have abnormal lymphocyte counts. Can anyone advise on the preferred...
15 August 2019 8,413 2 View
I am looking to stimulate PBMCs in vitro and to look at intracellular cytokine expression of IL-10 and TGF-b in T cell subsets. I have a couple of questions: 1. Most protocols I have seen use...
20 October 2017 8,779 6 View
I am looking to stimulate negatively isolated CD4+T cells in vitro and the main cytokines of interest are IL-10 and TGF-b. My questions are: 1. What should be my starting count (of CD4+ T cells)...
12 October 2017 3,060 3 View
Hi all, I am trying to isolate Tregs for further downstream analysis - in vitro stimulation and cytokine analysis. I have been using the Miltenyi Treg (CD4+CD25+) protocol which involves two...
06 September 2017 8,549 6 View
I am looking to expand and activate isolated T cells in vitro and then measure secreted cytokines using Luminex (mainly IL10, IFN gamma, TGB beta, TNF alpha. There seems to be various protocols...
16 June 2017 1,677 8 View
I am looking to do some in vitro experiments using IVIG but not sure what to use as my control. I have seen a paper where purified IVIG from a single donor was used and another paper where the Fab...
31 May 2017 3,160 0 View
I am looking to evaluate the suppressive function of regulatory T cells in children. Does anyone have a protocol that they could share? Thank you.
06 September 2015 3,920 7 View
