Mikkel Christensen-Dalsgaard

2 Questions 6 Answers 0 Followers

Questions related from Mikkel Christensen-Dalsgaard

Will an azide react with an alkyne without the presence of the catalyst (Cu+) at room temp?

My question is whether I can expect a significant "background" in the classic copper-catalyzed azide-alkyne cycloaddition reaction? In my test setup, I detect a reaction between my alkyne and...

15 July 2015 6,079 5 View

Alkaline degradation of RNA: which buffer and temperature should I use to completely degrade RNA in a sample?

1. I want to degrade all RNA in an sample, without damaging the DNA in the same sample. What buffer (pH), temperature and time should I use? 2. I want to preserve the 3' cyclic phosphate that is...

13 May 2015 6,225 7 View