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Questions related from Mike Wagenbach
I have a non-human mammalian cell line (from a deer) that grows fairly well but is not immortalized. I want to do a CRISPR HDR integration and isolate clones to store for future experiments,...
3 weeks ago 3,761 3 View
I found the following recipe at Abcam and converted it to 5x to make a stock solution. Running buffer (Tris-Glycine/SDS) 25 mM Tris base 190 mM glycine 0.1% SDS Check the pH and adjust to...
01 June 2024 1,697 2 View
Anyone know of a buffer that will work adequately for PCR with Takara's Primestar GXL? I have tons of enzyme but am out of buffer. Takara will sell a pack of extra buffer tubes, but it won't...
13 September 2023 2,497 1 View
Long ago, I began using the program DNAStrider for my subcloning sequence analysis because it was simple, free, and did almost everything I wanted and not much that I didn't want. When Strider no...
22 May 2023 1,783 0 View
I made an antiserum in a rabbit immunized with a part of my protein of interest (about 150 KD) fused to Maltose Binding Protein. (The protein fragment alone forms inclusion bodies in bacteria, so...
31 October 2019 8,402 3 View
