Miguel Hernandez Madrigal

2 Questions 5 Answers 0 Followers

Questions related from Miguel Hernandez Madrigal

What are these unexpected bands in PCR (around 100bp) [NOT primer dimers]?

I ran a PCR of reverse transcribed RNA samples using a primer combination that should amplify two splice variants of a gene. One of the splice variants should be 399bp in size, while the other...

09 September 2019 1,325 6 View

Is Click-IT AHA compatible with ELISA detection?

I am trying to measure protein synthesis rate of a particular secreted protein that is very hard to see in western blot. However we have a good stablished method for ELISA detection. My question...

07 July 2019 8,474 3 View