Miguel Fernández de Ullivarri

4 Questions 7 Answers 0 Followers

Questions related from Miguel Fernández de Ullivarri

Smears in sds-page - precipitation? Glycosilated proteins?

Hi all, I need some help. I am trying to purify and characterize an extracellular killer toxin (protein) from the yeast W. anomalus. But I am having trouble with SDS-PAGE analysis of the purified...

05 May 2017 8,786 3 View

Acidic Polyacrylamide gels?

is there a protocol for preparation of native polyacrylamyde gels at pH 4 or below?? Thanks

05 May 2016 3,216 3 View

Does previous fixing of gel affects the protein transference from gel to membrane?

I want to perform a transference of proteins from a gel fixed with acetic acid:ethanol:H2O (no coommassie) to a nitrocelulose membrane using a transblot SD (semi-dry, Bio Rad). Does fixing affects...

03 March 2016 8,184 3 View

Does anyone work with killer antibodies and/or killer peptides?

I would like to exchange some ideas and protocols advices on this area for the development of vaccines against different pathogens.

01 January 1970 5,608 0 View