Miguel Martín Serrano

2 Questions 2 Answers 0 Followers

Questions related from Miguel Martín Serrano

Why do I see no GFP expression in my cells after CRISPR/Cas9 if the vector includes it?

Hello everyone! I will summarize my situation: I am performing KO using CRISPR/Cas9 for PTEN in Prostate Cancer cells (DU145 and 22RV1). I am using a commercial vector that includes the guides,...

06 February 2025 3,787 1 View

How to see ZIP9/SLC39A9 by western blot?

Hello, I am trying to see and quantify by western blot the ZIP9 or SLC39A9 protein in Jurkat and Raji cells. I am using the parental lines and ZIP9-knockdown lines (by qRT-PCR I have observed an...

16 December 2024 5,009 3 View