Miguel Hernandez Madrigal

2 Questions 8 Answers 0 Followers

Questions related from Miguel Hernandez Madrigal

What are these unexpected bands in PCR (around 100bp) [NOT primer dimers]?

I ran a PCR of reverse transcribed RNA samples using a primer combination that should amplify two splice variants of a gene. One of the splice variants should be 399bp in size, while the other...

06 September 2019 2,731 6 View

How to produce clearer zymograms and solubilize cell extract samples?

I am currently trying to analyse pro-MMP9 and active MMP9 levels in cell culture media and cell extracts through zymography, but I have encountered two major issues I cannot fix.1.- My zymogram...

01 March 2017 9,395 4 View