Michelle Yoon

2 Questions 1 Answers 0 Followers

Questions related from Michelle Yoon

How to obtain cell pellet from 1x PBS wash 2 after 70% ethanol fixation?

I am handling HL60 cells and am intending to stain with PI to study cell cycle. However, I am unable to obtain a pellet from the 2nd PBS wash after ethanol fixation. 1. What has gone wrong? 2....

15 May 2025 4,068 0 View

Are multiple bands expected from overlap PCR?

Have performed PCR of Spycatcher gene (~339 bp) and Pal gene (~498 bp) with their respective primers, P1 forward and P2 reverse, and P3 forward and P4 reverse, respectively. Ran a 1% agarose gel...

24 July 2023 9,582 0 View