Michelle Kickham

5 Questions 13 Answers 0 Followers

Questions related from Michelle Kickham

Cell Culture infection ONLY APPARENT after transfection?

Hi Guys For the last couple of months my lab has been riddled with infections. All cell lines. It is odd though in that the infection is only visible the day after transfection with either CaP or...

09 September 2018 2,485 5 View

Acid interfering with Luciferase Reporter Gene Assay?

I am having some difficulties with my luciferase reporter gene assays. The cells are being transfected with peptides that are dissolved in 25% acetic acid (stock). The final acetic acid...

07 July 2017 5,944 4 View

Increasing retention time of a sample in HPLC?

Hey Everyone I am having trouble collecting my sample from HPLC due to it's very short retention time of under two minutes. The way the machine is set up, it cannot be collected this early...

06 June 2017 6,560 13 View

Syringe of Semi-Prep HPLC not reaching the bottom of sample vials, and leaving behind .5ml sample. How to increase travel depth of syringe?

Hey there, Another HPLC question! I am using a Gilson GX-271 Semi Prep HPLC (using the Trilution software) and I am having some issues with it. The main one at the moment is the needle/syringe...

08 August 2016 8,238 7 View

Process of moving from analytical HPLC to Semi-Prep HPLC - advice/tips on how to do it? Using Trilution software and Gilson GX271 model?

I'm in the process of purifying peptides using a Semi-Prep HPLC (Gilson) and I'm looking for any advice on how to switch over from analytical to Semi-Prep using the Trilution Software (I have the...

07 July 2016 7,107 10 View