Michele Tiberi

3 Questions 3 Answers 0 Followers

Questions related from Michele Tiberi

Doing EMSA with a protein that binds DNA as a dimer, would you consider the concentration of the dimer or the monomer?

Hi everyone, I'm working on a transcription factor which binds as a dimer its target DNA, composed by two palindromic half-sites (5'-TTGGCnnnnnGCCAA-3'). Talking about Electrophoretic Mobility...

25 November 2022 884 1 View

Do you know any molecule known to promote protein/DNA complex formation?

Hi everyone, I'm trying to crystallize a truncated DNA-binding domain of a transcription factor (Nuclear Factor I X) but its DNA-binding affinity is not optimal, therefore the complex is not very...

28 July 2022 5,054 3 View

Why does my protein precipitate after cleavage with thrombin in solution?

Hi, I am currently working on an eukaryotic transcription factor called Nuclear Factor I X. I generated a truncated construct of this protein to remove the intrinsically disordered C-terminal...

21 July 2022 7,533 5 View