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Questions related from Michal Zielinski
I Have Recently I have begun a project on expression and purification of a cytoplasmic protein. When running analytical Superdex 200 the protein elutes in the void volume despite the monomer being...
15 November 2023 6,755 1 View
Hi everybody, I am trying to pack a Sephadex G25 column in a HiScale 16 column. I have been reproducing my runs and I consistently get the asymmetry to fall within the allowable threshold of...
14 June 2022 8,269 0 View
I have solved a 3D structure of a protein with a ligand which seems to sit in a VERY strained conformation. I would like to quantify it by directly comparing conformational energy profile of...
24 September 2021 6,103 1 View
Dear all, Recently I have had a pleasure to work on some high resolution X-ray data. I have solved one structure of around 270 amino acid long enzyme which has been a breeze in this scenario, and...
23 March 2020 5,183 3 View
Dear All, I am currently working on a project based around an antibiotic resistance enzyme and I got some really interesting results. I am looking to confirm whether the enzyme under...
20 June 2019 7,708 4 View
I am currently working on a esterase and I am thinking it might have a Zn ion bound to it, but another evidence is pointing towards it being Ca2+. Is there a way in which it would be possible to...
11 April 2018 9,607 2 View
Dear Everyone, I have recently taken up a very interesting project on an enzyme which behaves very interestingly. When I first solved the structures of the enzyme (res. 1.4 Å), it appeared that...
01 January 1970 3,502 3 View
