Michal Zielinski

6 Questions 1 Answers 0 Followers

Questions related from Michal Zielinski

FPLC Column Packing, why is reduced plate height failing?

Hi everybody, I am trying to pack a Sephadex G25 column in a HiScale 16 column. I have been reproducing my runs and I consistently get the asymmetry to fall within the allowable threshold of...

14 June 2022 8,265 0 View

How to calculate strain of small molecule?

I have solved a 3D structure of a protein with a ligand which seems to sit in a VERY strained conformation. I would like to quantify it by directly comparing conformational energy profile of...

24 September 2021 6,092 1 View

High R-free despite great LLG and TFZ values, can anyone advise?

Dear all, Recently I have had a pleasure to work on some high resolution X-ray data. I have solved one structure of around 270 amino acid long enzyme which has been a breeze in this scenario, and...

23 March 2020 5,180 3 View

Constitutive expression of a gene in E. coli - In search of plasmid?

Dear All, I am currently working on a project based around an antibiotic resistance enzyme and I got some really interesting results. I am looking to confirm whether the enzyme under...

20 June 2019 7,703 4 View

How to detect presence of metal ion in a protein?

I am currently working on a esterase and I am thinking it might have a Zn ion bound to it, but another evidence is pointing towards it being Ca2+. Is there a way in which it would be possible to...

11 April 2018 9,604 2 View

Active site occupancy, why is n=0.5? (ITC and X-ray crystallography)

Dear Everyone, I have recently taken up a very interesting project on an enzyme which behaves very interestingly. When I first solved the structures of the enzyme (res. 1.4 Å), it appeared that...

01 January 1970 3,499 3 View