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Questions related from Michal Levy
I need to isolate macrophages from PBMC for RNA seq. The macrophages will be isolated 5 hours after activation (with Zymosan,through TLR 2/6). What would be the best method? And if I isolate them...
02 March 2023 9,790 2 View
Our lab has been working with fresh PBMC for decades. We separate them from buffy coats and seed them, incunate them overnight, then we treat them with inducers (SEB, LPS and others) and measure...
20 April 2020 9,662 0 View
Does anyone use the TC20 automated cell counter to count PBMC?
06 September 2017 9,289 5 View
I isolated a large amount (I hope) of exosomes and I need to quantify the membrane proteins with a Bradford assay before doing a WB. I started off with 70 ml of conditioned medium which I managed...
12 July 2016 8,396 8 View
I want to do a BCA protein microplate assay but I only have a 595 nm filter, while the manufacturer states that it should not be more than 590 nm. Has anyone been successful with a 595 nm filter?
06 April 2016 3,542 3 View
