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Hi there , I'm trying to do tumorigenesis recently . 1 week after injecting 2 X 10^6 cells in nude mice I observed small bumps under mice skin, and I thought they are tumors. But in the second...
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When I detect phosphorylation and acetylation there is always non-specific band around 90kd which I suspect is caused by the 5% milk blocking buffer ( other antibody do not show similar bands) ,...
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Is there anyone isolating exosomes from mouse embryonic stem cell ? How much proteins would you expect after ultracentrifuge ? The protein yield I got was extremely low, sometimes it's no more...
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