Michael Shaw

3 Questions 5 Answers 0 Followers

Questions related from Michael Shaw

Anyone facing a problem of " decreasing tumor " in nude mice?

Hi there , I'm trying to do tumorigenesis recently . 1 week after injecting 2 X 10^6 cells in nude mice I observed small bumps under mice skin, and I thought they are tumors. But in the second...

09 September 2016 3,244 9 View

What's the best blocking buffer for acetylation or phosphorylation protein detection ? BSA, milk or gelatin ?

When I detect phosphorylation and acetylation there is always non-specific band around 90kd which I suspect is caused by the 5% milk blocking buffer ( other antibody do not show similar bands) ,...

11 November 2015 300 3 View

How much exosome protein can I expect after isolation from mouse embryonic stem cells?

Is there anyone isolating exosomes from mouse embryonic stem cell ? How much proteins would you expect after ultracentrifuge ? The protein yield I got was extremely low, sometimes it's no more...

11 November 2014 7,724 4 View