Michael jeremy Shapiro

2 Questions 76 Answers 0 Followers

Questions related from Michael jeremy Shapiro

Measurement of heme in cultured cells?

I want to determine if a treatment alters heme synthesis in cultured fibroblasts. I have found several papers in which this was done using a QuantiChrome Heme assay kit, which I have. The papers...

07 July 2016 6,878 4 View

If I use Gibson Assembly to insert a fragment into a vector cut with EcoRI, will I get mostly reclosed vector?

I need to subclone a gene into an unusual vector that has only EcoRI at the insertion site. I don't have a construct in which my gene is flanked by EcoRI sites, so I will have to PCR amplify it...

11 November 2015 2,754 6 View