Michael Plank

3 Questions 20 Answers 0 Followers

Questions related from Michael Plank

How can I use information from different protease digests for PTM localization?

Hi, it should be possible to enhance your confidence in PTM site localization by combining information from digests with different proteases, e.g. by considering where the peptides overlap and...

03 March 2015 4,367 3 View

LC-MS/MS for low abundance peptide sampling?

I was wondering if anyone knows a good strategy to analyse a low abundance sample in proteomics. More specifically, I have digests from slices of a gel lane where I see proteins only by silver...

03 March 2014 6,431 18 View

How would you recommend to troubleshoot a proteomics identification experiment (data-dependent MS)?

I have a sample where I can assign only 20% of spectra (instead of normally >50%). What can I do to find out where to improve the experiment? The total ion chromatogram looks normal as far as I...

01 January 2014 5,571 15 View