Michael Lingelbach

4 Questions 14 Answers 0 Followers

Questions related from Michael Lingelbach

Why is my primary mouse neuron cell yield so low when doing a BSA cushion purification step?

Hi all, I've had a lot of success in the past generating postnatal (p0) primary mouse neuron cultures, in part due to the help and advice of the researchgate community (edit: shoutout to Drs....

10 October 2016 4,840 10 View

How do you apply VALAP without making a mess?

Hi All, I've switched to using prolong diamond and VALAP as a sealant for my coverslips (from using vectashield and nail polish) but I've discovered it's quite tricky to apply VALAP without...

04 April 2016 1,387 3 View

What is the most appropriate buffer for dissecting and dissociating postnatal mouse cortex in the presence of DNAse?

Hi All, I am currently using a modification of the Hilgenberg and Smith (Jove 2008) protocol for generating primary neuron cultures from p0-p2 mouse cortex. I have had success recording from these...

02 February 2016 2,435 16 View

Can anyone help me with an issue I am having comparing sanger sequencing data to ClinVar and other NCBI databases?

I'm new to bioinformatics (not my field) and am relying on GUI based programs to handle data processing (for now). Currently, I'm using Geneious to compare sanger sequencing data from one of our...

06 June 2014 2,174 2 View