Michael Adams

3 Questions 11 Answers 0 Followers

Questions related from Michael Adams

How do I align a sequence set to one reference sequence?

I am currently trying to find homologues of a protein I am working with, but BLAST has been giving me nothing useable. I have now found a dataset of 1500 protein sequences of potential candidates...

06 June 2019 6,553 3 View

Akta purifier UV unstable. What could be the cause?

Hey everyone. I am currently purifying a protein using a S200 10/300 GL size exclusion column on an Akta purifier. I injected the protein and the UV is going crazy. The protein was filtered and...

06 June 2019 1,476 3 View

Gallium (III) IMAC - can FPLC columns be reused?

I am currently working on purifying a phosphoprotein of approx. 25 kDa in length and a pI of 6. In order to purify the phosphoprotein using FPLC, we used a Gallium (III) HiTrap column by GE and an...

10 October 2017 321 6 View