Why are the Ct values for my endogenous control (18s) so high and/or variable for the qPCR I'm performing on whole blood samples?

I'm performing qPCR on bovine whole blood samples collected 9/2021-11/2021 and stored/thawed a handful of times before initial PCR testing for anaplasmosis locally and then for BLV via PCR at...

01 July 2023 5,583 3 View