Michael Swanson

2 Questions 14 Answers 0 Followers

Questions related from Michael Swanson

Can I move the first Met away from the rbs and still get good expression in pET24a?

I am using pET24a and I would like to use the NheI and XhoI sites to add our gene. If I use the NheI site at the N-terminal, the first methionine will be from the vector itself, and not the gene...

29 November 2016 2,550 6 View

How do I optimize the expression of unstable protein?

I have been trying to express a mammalian mitochondrial protein that is ~100kD. The gene is in the pET47b vector and I have been using the Rosetta2 (DE3) strain. I have done SDS-PAGE/westerns to...

01 July 2015 7,886 15 View