Michael jeremy Shapiro

3 Questions 51 Answers 0 Followers

Questions related from Michael jeremy Shapiro

Measurement of heme in cultured cells?

I want to determine if a treatment alters heme synthesis in cultured fibroblasts. I have found several papers in which this was done using a QuantiChrome Heme assay kit, which I have. The papers...

01 July 2016 1,180 3 View

If I use Gibson Assembly to insert a fragment into a vector cut with EcoRI, will I get mostly reclosed vector?

I need to subclone a gene into an unusual vector that has only EcoRI at the insertion site. I don't have a construct in which my gene is flanked by EcoRI sites, so I will have to PCR amplify it...

05 November 2015 10,072 5 View

Would an N terminal tag cause problems with overexpressing a transmembrane protein?

I recently purchased an expression construct from GenScript in order to overexpress a FLAG tagged protein in mammalian cells. The construct is based on pcDNA3 and has a Met, the FLAG epitope, and...

22 October 2015 6,437 4 View