Michael Ronzetti

2 Questions 10 Answers 0 Followers

Questions related from Michael Ronzetti

Does 6M guanidine HCl carry any risk of protein modification after extended incubation like urea (carbamylation)?

I'd like to perform a SEC step prior to on-column refolding of a protein I am expressing/purifying but am worried about the extended time the protein will be in the presence of urea (and...

18 August 2023 5,201 3 View

What is the smallest protein tag with high-affinity small molecule binders/protein-protein interaction?

Is there any system with small molecule binders and a short protein tag that is higher affinity than 6xHIS-NTA?

01 January 1970 7,904 3 View