Michael Ronzetti

3 Questions 12 Answers 0 Followers

Questions related from Michael Ronzetti

Does 6M guanidine HCl carry any risk of protein modification after extended incubation like urea (carbamylation)?

I'd like to perform a SEC step prior to on-column refolding of a protein I am expressing/purifying but am worried about the extended time the protein will be in the presence of urea (and...

18 August 2023 5,307 3 View

Analysis of Microscale Thermophoresis experiments. When to trust TJump vs. Thermophoresis only vs TJump + Thermophoresis?

Thank you in advance for reviewing my issue. I am wondering when it is appropriate to employ a different analysis than Nanotempers default, which is a combination of the TJump and...

26 January 2017 7,678 0 View

What is the smallest protein tag with high-affinity small molecule binders/protein-protein interaction?

Is there any system with small molecule binders and a short protein tag that is higher affinity than 6xHIS-NTA?

01 January 1970 7,960 3 View