Michael Lingelbach

2 Questions 7 Answers 0 Followers

Questions related from Michael Lingelbach

How do you apply VALAP without making a mess?

Hi All, I've switched to using prolong diamond and VALAP as a sealant for my coverslips (from using vectashield and nail polish) but I've discovered it's quite tricky to apply VALAP without...

01 April 2016 7,998 2 View

What is the most appropriate buffer for dissecting and dissociating postnatal mouse cortex in the presence of DNAse?

Hi All, I am currently using a modification of the Hilgenberg and Smith (Jove 2008) protocol for generating primary neuron cultures from p0-p2 mouse cortex. I have had success recording from these...

23 February 2016 5,596 15 View