Michael-Jon Rosslee

3 Questions 2 Answers 0 Followers

Questions related from Michael-Jon Rosslee

Detection of TB in a BMDM assay using GFP or separation of bacteria from cells?

Morning I currently have the dilemma of detecting M.tuberculosis in a BMDM assay looking at the efficacy of drugs in killing intracellular M.tuberculosis. Ideally, it would be beneficial if I...

05 July 2016 9,982 3 View

Use of glass beads in generating single cell suspension of Mycobacterium tuberculosis?

Hi all I am currently working with Mycobacterium tuberculosis and need to disrupt Mtb clumps/generate single cell suspension. Stock is defrosted, centrifuged, the supernatant removed and the...

11 April 2016 7,668 5 View

Why have Non-ionic surfactant vesicles (NIVs) not been more common place? I.e. What have been their limitations?

Am currently working with NIVs and am struggling to understand why, if discovered in the 1970s and first utilised as a drug delivery system (DDS) in 1985 (Chemotheraphy), why has their use as a...

16 March 2016 7,789 2 View