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Just got a new LiCOR Odyssey Fc imaging system, which can do fluorescence multiplexing in near IR wavelengths. Total protein detection has become the normalization technique of choice (better...
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I am selecting for stable transformants in an N2a cell line. The construct looks like this: EF1a-gene of interest-IRES-eGFP...SV40-EM7-blasticidin resistance. I seem to be getting good...
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I am running hand cast 12% bis-tris gels using MES buffer (novex formulations) at 100v. Every so often, towards the end of the run I notice a warping of the gel at the bottom corners which...
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