Michael David Hoos

4 Questions 6 Answers 0 Followers

Questions related from Michael David Hoos

Has anyone compared the total protein stains, MemCode (Pierce) with Ponceau S, and Revert (LiCOR)?

Just got a new LiCOR Odyssey Fc imaging system, which can do fluorescence multiplexing in near IR wavelengths. Total protein detection has become the normalization technique of choice (better...

08 March 2016 4,912 5 View

Does anyone know of an antibody that is specific for the GOLLI region of GOLLI-MBP proteins?

I need to distinguish GOLLI proteins from their related myelin basic protein isoforms. It seems that most GOLLI-MBP antibodies are specific for the MBP containing regions of the proteins and...

30 December 2015 9,255 0 View

What are effective inhibitors of methylation for use in cell culture?

I am selecting for stable transformants in an N2a cell line. The construct looks like this: EF1a-gene of interest-IRES-eGFP...SV40-EM7-blasticidin resistance. I seem to be getting good...

20 September 2014 5,675 3 View

Has anyone ever experienced warping/melting of gels in a biorad mini-protean gel box?

I am running hand cast 12% bis-tris gels using MES buffer (novex formulations) at 100v. Every so often, towards the end of the run I notice a warping of the gel at the bottom corners which...

22 November 2013 8,932 1 View