Why are only 1 percent of cells dying after selection in a culture transduced with an MOI of .3?

I am doing primary fibroblast cell culture and transduced my cells with a lentivirus with 10^7 TU/mL. I added 7.5 uL of virus to about 250,000 cells to have an MOI of about .3. Of note polybrene...

08 September 2021 7,020 3 View