Michael Firer

11 Questions 26 Answers 0 Followers

Questions related from Michael Firer

Where can I purchase the 5TMM myeloma cell line?

We are looking to set up the 5TMM myeloma model in C57Bl/6 mice. Where can we obtain the cell line? Thanks

29 June 2025 1,031 2 View

How to collect and isolate oral mucosal epithelial cells from live mice?

We are looking to develop a non-invasive method to obtain normal epithelial cells, initially in a mouse model. Methods are available for isolating the cells from euthanized mice. Does anyone know...

25 December 2024 7,593 4 View

What statistic test should I use?

I have data on control and test groups of mice at several time points. I want to test the significance in a change in index of control over test groups at the different time points. What test...

17 September 2024 834 4 View

Do you have a protocol for stimulation of mouse B cells by PWM?

Looking for a protocol for the stimulation of B cells with pokeween mitogen

23 June 2024 4,961 4 View

Any suggestions to help grow small cell lung cancer cells?

We are working with Small Cell Lung Cancer cell lines, H209, H146, H69. These cells usually grow in floating clumps. Recently we are having problems maintaining their viability. After a few days...

24 April 2022 10,004 2 View

Can T cell activation in vitro work adequately by just using PBMCs?

I am setting up to test antigen specific T cell responses in patient blood. Some protocols just culture whole PBMCs with antigen (presumably relying on blood monocytes to differentiate into...

07 April 2020 594 3 View

Can the H-2 haplotype of a cell be changed?

Hi, We are studying T cell heterogeneity in immunotherapy for cancer. I would like to know if the same mouse T cell clones reactive to a H-2 compatible tumor cell also react to the same type of...

13 December 2019 4,753 0 View

How to culture human bone marrow lymphocytes?

I am looking for a method to culture and stimulate T cells in human bone marrow samples

19 December 2018 3,503 3 View

Protocol for purifying anti-idiotypic antibodies?

Hi everyone, I am looking for a good protocol to purify mouse IgG anti-idiotypic antibodies. Any suggestions? Thanks

05 March 2018 4,643 5 View

What tool do you recommend for analysing peptide sequences?

I have a series of peptide sequences and I'm looking to see if there is a consesnsus sequence or motif. What tools do you recommend?

04 July 2016 8,242 8 View

Trouble with leaky ELISPOT plates

We are developing an ELISPOT to measure antigen specific mouse B cells. We are using a standard protocol in which PVDF microplates are activated with 70ul ethanol then washed. Antigen solution is...

23 October 2012 9,489 10 View