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Questions related from Michael Essien Sakyi
I want to prepare 0.05 M bicarbonate carbonate, pH 9.6 as coating buffer for ELISA. Could anyone help me with right recipe, procedure, and or reference in the preparation? Thank you in advance.
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My His-tagged purified protein contains 50mM sodium phosphate, 300mM NaCl, and 8M urea at which the protein was eluted at pH 4.9 and pH 4.3. What should be the ideal dialysis buffer composition to...
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During the sample preparation of cell lysate, add cell lysis buffer, agitate the cells, centrifuge, and recover and aliquot supernatant. What is the procedure/protocol for cell pellets preparation...
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I am uncertain about using HEK293 cells and MDCK cells for transfection using a lipofectamine reagent with a GFP tag plasmid. Both cell lines are mammalian cells. Please your response is appreciated.
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