Michael Cukan

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Questions related from Michael Cukan

Can someone name examples of functional Cys2His2 domains that fold into the characteristic ββα fold in the absence of zinc?

The Wikipedia page on zinc fingers states that such domains exist; salt bridges reportedly take the place of zinc. However, there is no citation! The only example of a natural zinc-stabilized DNA...

08 July 2016 9,763 1 View

Is engineering of msDNA retron sequences possible for the production of small custom dsDNA?

I require large amounts of small (16bp) dsDNA molecules for zinc finger binding studies. This is the major bottleneck in my research since the large amounts are expensive to purchase synthesized...

29 July 2015 4,868 3 View

PhD programs for a biotechnology career: is there a downside to a doctorate from an engineering program?

I want to further my career in biotechnology with a PhD. I've applied to a number of programs including a standard Molecular Cell Biology program as well as an Engineering program in the same...

24 January 2013 1,945 3 View

Heparin LC column memory: use of detergent to get complete elution?

I am trying to develop a heparin affinity column method using a 100mM to 1M NaCl step elution. My protein of interest actually elutes at a 0.5M NaCl step, but though it elutes in a nice, sharp...

06 September 2012 2,924 3 View