Michael Baumgartner

2 Questions 6 Answers 0 Followers

Questions related from Michael Baumgartner

Is the native 5'UTR present in UAS-driven proteins generated from cDNA?

I'm trying to determine if the UAS-driven protein construct I am using in Drosophila possesses the native 5'UTR, or if the process of generating the flies swaps out the 5'UTR for another. The...

19 March 2018 1,495 3 View

I'm having problems with SYTO RNAselect

I've been trying to use SYTO RNAselect to label Drosophila imaginal wing discs, but the dye appears to be binding DNA, not RNA. I fix in cold methanol (-20C) and counterstain with Hoechst, and the...

15 June 2017 1,034 36 View