Micaela Riscado

2 Questions 1 Answers 0 Followers

Questions related from Micaela Riscado

For PCR optimization, can I use different primer concentrations for the target gene and the reference gene?

In qPCR optimization I have a gene that has low expression and my samples dilution (1:3) gives me a calibration curve with low R^2. And I have another gene that gives me a nonspecific band...

17 December 2023 2,788 2 View

For PCR optimization, can I use different primer concentrations for the target gene and the reference gene?

In qPCR optimization I have a gene that has low expression and my samples dilution (1:3) gives me a calibration curve with low R^2. And I have another gene that gives me a nonspecific band...

01 January 1970 5,742 3 View