Mesele Belete

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Questions related from Mesele Belete

Bioinformatic Assembly of a Tripartite Viral Genome: Merged Contig of Alfalfa Mosaic Virus (AMV) RNA1 and RNA2?

Hello everyone, I'm working on a viral genomics project and have encountered an interesting assembly result that I'd appreciate some professional advice on. I have sequenced the genome of an...

last month 3,721 0 View

How to submit RNA-seq data to NCBI?

I have performed a virome analysis on a single plant (host) and found raw viral sequence data generated by High Throughput Sequencing (HTS). The reviewers of the journal have requested that I...

07 February 2024 4,642 1 View

How can I deposit multiple viral sequences, discovered in a single plant through virome analysis, into the NCBI as a BioProject?

I conducted a virome analysis on a single plant and discovered various viral sequences within it. Can anyone guide me on how to deposit these multiple sequences (different viral sequences) at once...

02 February 2024 3,093 0 View

How to design 5'RACE primer?

I have know sequence of mRNA. but i need to amplify the unknown region of 5'end ? to do this that needs GSP1(reverse) and GSP2 primer(forward). my kit protocol states your GSP2 primer is anneals...

22 October 2020 7,260 2 View