Mervi T Hyvönen

5 Questions 10 Answers 0 Followers

Questions related from Mervi T Hyvönen

How to differentiate 51 and 55 kDa protein isoforms?

I have observed expression of two splice variants, which should code protein isoforms of 51 and 55 kDa. I have antibody for the protein, but is there any conditions for SDS-PAGE to differentiate...

11 June 2019 1,612 9 View

Enzyme pH optimum in vitro vs. subcellular localization

Why does a cytosolic enzyme (pH~7.4) have an pH optimum of 9.0 in vitro (recombinant protein)?

10 June 2017 571 11 View

In vitro translation - 14C-leucine vs 35S-methionine exposure difference?

I'm using 35S-methionine to label in vitro translation products, but due to the short half-life of the label I'm wondering whether I could substitute it with 14C-leucine. Promega and other...

16 August 2016 7,214 1 View

How to facilitate heterodimer formation?

I'm having in vitro-translated protein (reticulocyte lysate), which is homodimer, and its other in vitro translated isoform. Just mixing them together does not allow heterodimer formation. How do...

08 April 2016 6,051 5 View

Hormone content of FBS? Insulin depletion from FBS?

Does anyone know where to find the list of average concentrations of hormones in FBS? I know that insulin is about 40 pM in 5% FBS, but what about the other hormones? And does anyone have a...

23 April 2013 3,129 7 View