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Questions related from Mervi T Hyvönen
I have observed expression of two splice variants, which should code protein isoforms of 51 and 55 kDa. I have antibody for the protein, but is there any conditions for SDS-PAGE to differentiate...
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Why does a cytosolic enzyme (pH~7.4) have an pH optimum of 9.0 in vitro (recombinant protein)?
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I'm using 35S-methionine to label in vitro translation products, but due to the short half-life of the label I'm wondering whether I could substitute it with 14C-leucine. Promega and other...
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I'm having in vitro-translated protein (reticulocyte lysate), which is homodimer, and its other in vitro translated isoform. Just mixing them together does not allow heterodimer formation. How do...
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Does anyone know where to find the list of average concentrations of hormones in FBS? I know that insulin is about 40 pM in 5% FBS, but what about the other hormones? And does anyone have a...
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