Merve Vural

4 Questions 5 Answers 0 Followers

Questions related from Merve Vural

What is the problem in this SDS-PAGE result for secreted protein ?

Hi everyone, I am doing western blot for POMC/ACTH secreted from AtT20 cells. The problem is as seen in the picture I added. First 3 lanes are for lysates and not a problem. But the next lanes...

10 April 2018 1,375 6 View

What is the problem in this SDS-PAGE result for secreted protein ?

Hi everyone, I am doing western blot for POMC/ACTH secreted from AtT20 cells. The problem is as seen in the picture I added. First 3 lanes are for lysates and not a problem. But the next lanes...

04 April 2018 9,061 7 View

How to do western blot for POMC secreted from N2a cells?

I am doing secretion assay for Proopiomelanocortin (POMC) from N2a cells by using DMEM containing %0.1 BSA, w/o FBS, and by collecting medium in every 30 min. Then I stimulate the cells with KCl....

06 February 2018 6,169 3 View

How to do western blot for POMC secreted from N2a cells?

I am doing secretion assay for Proopiomelanocortin (POMC) from N2a cells by using DMEM containing %0.1 BSA, w/o FBS, and by collecting medium in every 30 min. Then I stimulate the cells with KCl....

02 February 2018 1,082 3 View